KCB Lions fell 22-28 at home to the visiting Mwamba RFC, their third home loss in the Kenya Cup this season.
Over in Kakamega, defending champions KCB Cubs beat West Kenya 22-10 to qualify for the Eric Shirley Semis on a 56-27 aggregate, having won the first leg in Nairobi 34-17.
In football's Kenya Premier League, AFC Leopards edged out Gor Mahia 1-0 at the Nyayo Stadium.
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Cavendish targets Merckx Tour de France record - This year's tour starts in the German city of Duesseldorf on Saturday.
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